钒钼黄比色法测定食品中磷的研究

建立了钒钼黄比色法测定食品中磷的检测技术 ,该法通过消解液酸度的调节 ,显色剂的替换 ,应用钒钼黄比色法测定食品中磷的含量.当磷溶液浓度范围在[1mg/L～20 mg/L ]时 ,线性回归良好 ;回收率范围为94%～97% .用此法测定了国家标准参考物质 ,与国标方法测定食品中磷的含量进行了比对 ,结果表明 :测定结果与国标方法的测定结果比较无显著统计学差别 ,说明该方法可用于食品中磷的测定.     

Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.

Previous studies showed that the yellow seed color gene of a yellow mustard was located on the A09 chromosome. In this study, the sequences of the molecular markers linked to the yellow seed color gene were analyzed, the gene was primarily mapped to an interval of 23.304 to 29.402M. Twenty genes and eight markers’ sequences in this region were selected to design the IP and SCAR primers. These primers were used to screen a BC₈S₁ population consisting of 1256 individuals. As a result, five IP and five SCAR markers were successfully developed. IP4 and Y1 were located on either side of the yellow seed color gene at a distance of 0.1 and 0.3 cM, respectively. IP1, IP2 and IP3 derived from Bra036827, Bra036828, Bra036829 separately, co-segregated with the target gene. BLAST analysis indicated that the sequences of newly developed markers showed good collinearity with those of the A09 chromosome, and that the target gene might exist between 27.079 and 27.616M. In light of annotations of the genes in this region, only Bra036828 is associated with flavonoid biosynthesis. This gene has high similarity with the TRANSPARENT TESTA6 gene, Bra036828 was hence identified as being the gene possibly responsible for yellow seed color, in our research.     

丘陵红黄壤区施用399对“一种两收”再生稻根系和产量的影响

[目的]研究399对"一种两收"再生稻的影响。[方法]以适宜丘陵红黄壤种植的丰两优香1号、准两优527、宜优673再生稻品种为材料,分别设置喷施399和不喷施399(CK)2种处理,研究各处理对再生稻根系和产量的影响。[结果]结果表明:施用399使水稻在生育期上延缓成熟,且施后再生稻的总根系数量、白根数量、根鲜重、根干重、根体积大幅度增加,同时在两季总产量上准两优527、丰两优香1号和宜优673品种分别比对照增产6.39%、4.86%、5.94%。[结论]施用399有利于准两优527、丰两优香1号和宜优673再生稻品种再生根的发生。     

治疗仔猪黄白痢中兽药临床处方筛选

为了筛选出治疗仔猪黄白痢的最佳中药处方，确定其合理用药剂量和适用范围，试验将478头确诊为黄白痢的哺乳仔猪随机分为6组，分别用3种预选处方（处方一、处方二和处方三）、氧氟沙星、诺氟沙星、氧氟沙星与诺氟沙星联合用药进行治疗，比较预选处方与对照药物对仔猪黄白痢的治疗效果，筛选最佳的中药处方；并对每种中药处方按照低、中、高3种剂量进行用药，确定预选处方的合理用药剂量；按照发病特点进行用药。探讨预选处方对不同病症的适用性。结果表明：预选处方对仔猪黄白痢的治疗效果优于对照药物，且复发率显著低于后者；3种中药处方中，处方一的疗效最好，治愈率为82．9％，总有效率可迭84．7％，显著高于其他处方；处方一中剂量组的疗效最好，治愈率为88．9％，总有效率可达91．1％，显著高于其他剂量组，而且治愈后无复发现象。     

单桩张网菱形、方形网目网囊选择性研究

近年来,一种利用帆布作为扩张装置的新型单桩张网渔具在吕四渔场得到了快速发展。为了改善这种张网对主要渔获对象的尺寸选择性,进而评价其渔获性能以及对渔业资源的影响,使用套网试验法开展了不同网目尺寸(25 mm、35 mm和45 mm)的菱形和方形网目网囊尺寸选择性试验。通过对黄鲫(Setipinnataay)和小黄鱼(Pseudosciaena polyactis)渔获体长数据的选择性分析,结果显示,无论是菱形还是方形网目网囊,其50%选择体长L50和选择范围SR都随着网目尺寸的增大而增大(L50呈线性增长)。对比菱形和方形网目的选择性发现,对于黄鲫,50%选择体长L50差异不大,但菱形网目的选择范围SR大于方形网目;对于小黄鱼,方形网目具有更好的选择特性,即具有较大的L50(8%)和较小的SR(52%),但差异均不显著。菱形和方形网目对于不同体型鱼类的选择性差异以及渔民对方形网目网囊的抵触情绪等都使得在张网渔业中推广方形网目网囊值得商榷。     

Effect of dietary protein levels on growth,feed utilization and carcass composition of endangered bagrid catfish Horabagrus brachysoma (Gunther 1864) fingerlings

An 84‐day feeding trial was conducted to study the effect of different levels of dietary protein, 250 (P25), 300 (P30), 350 (P35), 400 (P40) and 450 g (P45) kg^?1 dry matter (DM) on growth, feed intake, feed utilization and carcass composition of bagrid catfish Horabagrus brachysoma fingerlings. Triplicate groups of fingerlings with mean initial body weight of 2.2 g were fed the experimental diets twice daily, till satiation, in 150‐L tanks supplied with flow‐through freshwater. Daily dry matter intake by the fingerlings decreased significantly (P < 0.05) when fed P25 diet, containing 250 g protein kg^?1. The highest body weight gain, specific growth rate (SGR) and protein efficiency ratio (PER), and the lowest feed conversion ratio (FCR) were observed in fish fed 350 g protein kg^?1 diet. The fish fed with P45 diet had the lowest (P < 0.05) carcass lipid content. The polynomial regression analysis indicates that H. brachysoma fingerlings require 391 g dietary crude protein kg^?1 diet.     

黄河三角洲台田间渗水池塘的水质变动规律分析

以黄河三角洲地区台田间渗水池塘为研究对象,分析了渗水池塘的水质因子变动规律。结果表明:不同池塘间水质因子差异较大,但均表现出水体盐度较高、氮磷等营养物质含量较低、K+离子含量相对较低、Ca2+离子和Na+离子含量相对较高及K+/Na+显著低于正常海水等特点。结果说明,黄河三角洲地区台田间渗水池塘的水质条件不适合直接进行水产养殖,应对水质调整后才可做养殖之用。     

彩色茧（金黄色）新品种金秋×初日永康试养初报

家蚕新品种金秋×初日除具有天然金黄色蚕茧性状外，其它各项经济性状与对照种秋丰×白玉相仿。但在气候比较恶劣的环境下，金秋×初日明显的表现出强健性高，发病率低，是一对能适合适度规模化省力化饲养，特别是在饲养条件比较差的环境下取得稳产的优良品种。     

引黄济津应急调水工程河北段输水能力计算

针对引黄济津应急调水工程河北段的输水能力进行了研究。渠段的输水能力是指渠段所能通过的最大入流量，即渠段的首端断面所能通过的最大流量。根据引黄济津应急调水工程近4年的实测数据，构建了适于干河床水流推进过程渗漏损失的改进模型、小水深情况下的糙率加大模型，并采用均匀试验优选方法对水力参数进行了反演，利用非恒定流模型对引黄济津河北段渠系输水能力进行了计算。结果表明，建立的渗漏损失改进模型、小水深情况下的糙率加大模型是合理的，反演得到的参数是精确的；由于在水流推进与涨水阶段渠床的非稳定渗漏起了较大作用，所以各渠段的输水能力在非稳定输水阶段比稳定输水阶段稍大。输水能力的计算结果可以为引黄济津未来几年的输水规划与调度提供具体的指导。     

大果沙棘杂交后代果实主要成分遗传变异倾向研究

本文以黑龙江省绥棱浆果研究所选育的"楚伊×蒙优1"、"楚伊×蒙优2"、"楚伊×蒙优3"、"楚伊×蒙优4"、"HS-21×蒙优1"、"HS-22×阿列伊"、"绥1×蒙优7"和"HS-20×阿列伊"8个大果沙棘杂交组合为研究对象,对杂交后代果实中糖、酸和黄色素成分的遗传变异倾向进行了初步研究.结果表明:以蒙古沙棘系列雄株为父本的杂交组合后代果实中糖含量呈低亲变异的趋向,以阿列伊为父本的杂交组合后代果实中糖含量在一定程度上呈高亲变异倾向.阿列伊作为父本优于蒙古沙棘系列雄株."高糖"母本楚伊杂交组合的F1代株系果实中糖含量均呈现出衰退趋势.8个沙棘杂交组合后代果实中酸含量呈现一定程度的高亲变异."HS-21×蒙优1"杂交组合后代果实中酸含量变异广泛.8个沙棘杂交组合后代果实中黄色素含量一定程度上亦呈高亲变异倾向.在"楚伊×蒙优4"杂交组合后代中进行选择,获得超亲株系的几率最大.